MOESM10 of HDAC inhibitors cause site-specific chromatin remodeling at PU.1-bound enhancers in K562 cells

Additional file 10: Figure S6. PU.1 depletion fails to block HDACi-induced gene expression changes in K562. (a) Boxplots of fold-change in RNA-seq expression for SAHA treatment of vector control-, shRNA 1-, or shRNA 2-transfected cells (n = 2 replicates) for genes that either increase or decrease significantly in vector control K562 (FDR < 0.05). (b) Heatmap of individual replicate expression values (FPKM values scaled across each row) for genes significantly differential following SAHA treatment in vector control K562. Note the distinction between all vehicle treated and all SAHA-treated samples and lack of distinction between PU.1 knockdown and vector control samples