Virgin Panx1^-/- mice have normal mammary glands.

<p>(A) Evaluation of body weights at 4 and 7 weeks revealed that pre-pubertal Panx1^-/- mice were significantly larger at 4 weeks compared to control mice. Values are mean body weights ± S.E.M. * P <0.05. (B, C) Mammary gland weight and normalized mammary gland weight were not significantly different in Panx1^-/- compared to Panx1^+/+ mice. Values are mean weights ± S.E.M. (D) Whole mount analysis with carmine alum staining revealed normal epithelial ductal architecture embedded in a well-developed stroma. (E) Quantification of ductal elongation revealed no significant differences in knockout and control mice. Values are mean lengths ± SEM. (F) Histological evaluation of hematoxylin and eosin stained glands revealed normal tissue architecture in virgin Panx1^-/- compared to control mice. N = 9. Scale bar = 50 um.