Panx1^-/- mice have normal mammary glands at parturition.

<p>(A) Evaluation of body weight, mammary gland weight and normalized mammary gland weight revealed that Panx1^-/- mice were similar to control mice at parturition. (B) Whole mount analysis revealed numerous alveoli filling the mammary fat pad. (C) Histological evaluation of haematoxylin and eosin stained glands revealed normal tissue architecture, a similar average number of alveoli and a similar average alveolar lumen area in Panx1 null mice compared to control mice. (D) Quantification of the average epithelial area as assessed with immunofluorescent analysis using pan-cytokeratin (green) revealed similar epithelial area in the mammary gland of Panx1^-/- compared with control mice. (E) Quantification of the average number of adipocytes as assessed with immunofluorescent analysis using perilipin (green) revealed a similar number of adipocytes in Panx1^-/- mice compared with control mice. Hoescht (blue) denotes nuclei. Bars are means ±S.E.M. N = 6. Scale bars = 50 um.