HPeV-1 infects the cell lines that do not express αVβ3 and αVβ6 integrins.

<p>(A) A549, HeLa, and SW480 cell lines were inoculated with HPeV-1 at MOI of 10, unbound viruses were removed followed by incubation at 37°C for 6 h, and processed for immunofluorescence staining and imaging (with a 20×objective). HPeV-1 particles and nuclei are shown in green and blue, respectively. (B) The receptor profile of A549, HeLa and SW480 cells was analyzed with flow cytometry. Cells were fixed, and cell surface integrins (αV, β1, αVβ3, αVβ6 and α5β1) were stained using specific antibodies, and 20 000 cells/antibody was measured by flow cytometry.