Heatmap of unsupervised hierarchical clustering of AVS data on the basis of 59 differentially methylated CpG sites.

<p>Unsupervised hierarchical clustering analysis is very popular in identifying methylation-defined patient sub-groups. Fig 1 displays CpG sites that are at least either 1.5 fold demethylated or 1.5 fold hypermethylated in the disease (AVS) condition (red colored squares) compared to normal subjects (blue colored squares). Differentially methylated CpG sites have been displayed in three clusters (row-wise). The figure also displays direction, fold change in disease, probe relationship and probe annotation of differentially methylated CpG sites. Red color in the heatmap indicates hyper DNA-methylation, and blue hypo DNA-methylation values.