Additional file 2: of A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2

Expression of progenitor markers in the hindbrain. A. Typical flow cytometry plots for 18HH hindbrain cells stained with the progenitor markers Transitin and CSPG antibodies. (b,e) Control samples stained with secondary Ab only. (c,f) Samples stained for Transitin and CSPG, respectively. B. Representative flat-mounted views of hindbrains of 15HH chick embryos stained of progenitor markers. Hindbrains were immunostained for Sox2 with Pax6 (a-c) and Transitin (d-f) (n = 10/marker). Red/green or merged channels are shown in images (a-f), respectively. Scale bars = 100 μm. (TIF 1062 kb