NK cells selectively kill splenic B cells from mice infected with <i>T</i>. <i>brucei</i> AnTat 1.1.

<p>eFluor 670-labeled B cells from naive mice or mice infected with <i>T</i>. <i>brucei</i> 8 days earlier (10⁵ B cells/well) were incubated at 37°C with or without NK cells from naïve mice or mice infected with <i>T</i>. <i>brucei</i> 9 days earlier to give ratios of between 50 and 0.25 NK cells/1B cell. After 3 hr incubation viable B cells were enumerated in each well. Results are presented as: relative viable B cell recovery, where 1 is the number of viable B cells remaining in cultures after incubation in the absence of NK cells. This was 8 x 10⁴ B cells from uninfected mice/well, and 5 x 10⁴ B cells from infected mice/well). The study was repeated 3 times with similar results.