Splenic B cell populations in uninfected and <i>T</i>. <i>brucei</i> AnTat 1.1-infected TCR-/- C57BL/6 mice.

<p>Splenic B cells in uninfected B6-TCR^-/- mice (n = 3) and B6-TCR^-/- (n = 3) mice that had been infected with <i>T</i>. <i>brucei</i> AnTat 1.1 for 10 days were stained for surface markers used to define transitional and mature B2 B cells as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005733#ppat.1005733.t001" target="_blank">Table 1</a> and analyzed using flow cytometry. Results are presented as individual and mean +/- 1SD numbers of: <b>(A)</b> Transitional 2+3 B cells/spleen, <b>(B)</b> MZB cells/spleen and <b>(C)</b> FoB cells/spleen. Significance (*p<0.05; **p<0.01) is determined using one-way ANOVA and Tukey’s HSD test comparing uninfected controls to infected individuals. Results are representative of 3 identical experiments. <b>D–G</b> Representative FACS plots of: <b>(D)</b> FoB and MZB spleen cells from an uninfected TCR^-/- C57BL/6 mouse, <b>(E)</b> FoB and MZB spleen cells from a day 10 infected TCR^-/- C57BL/6 mouse, <b>(F)</b> Transitional (AA4.1+, B220+) <b>s</b>pleen cells from an uninfected TCR^-/- C57BL/6, <b>(G)</b> Transitional (AA4.1+, B220+) <b>s</b>pleen cells from an a day 10 infected TCR^-/- C57BL/6 mouse.