FFA inhibits the migration of monocytes in vitro.

<p>Murine monocytes were isolated from the bone marrow of C56Bl6/J mice and co-cultured for 2 hours in transwell plates with TSEC (A) or AML12 (B) either resting or pre-treated with FFA and LPS for 18 hours. Cells were stained with the monocyte/macrophage markers CD11b, F/480 and Ly6C and the chemotactic index was calculated (#migrated cells in treatment wells/# migrated cell in the control well). (C) Representative histograms showing Ly6C expression on migrated cells. (D) The chemotactic index of Ly6C high and Ly6C low cells towards resting or FFA-treated TSEC. Plots represent the mean +/- SE of three experiments. *p<0.05.