Method for restriction digestion of the TB Control Plasmid, dPCR analysis using the QuantStudio 3D platform, dPCR primers and thermal cycling conditions information, dPCR partition volume and partition number and mean λ values. Results from stability testing of the materials and analysis of BCG/ASM and Total MTB Control materials by Xpert MTB/RIF end-user laboratories including instrument and assay information, extraction and qPCR methods used by qPCR end user laboratories and their reported Cq values. (DOCX 114 kb
Detailed assay protocol. Table S1. DNA sequences for the oligonucleotides used in the 2-marker blood...
Figure S1. Distribution of sample numbers in three TB databases. Number of isolates with available D...
Supplementary Material 3: Table S4 List of primers used in this study for the detection of target ge...
Table S1. Investigating the presence of rpoB and 5KST diagnostic targets in M. tuberculosis complete...
Background: Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being u...
Background: Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being us...
Table S1. Taqman Gene Expression Assays used for qt-PCR. Table of Taqman primer probe sets for assay...
Comparison of conventional procedures with single-step & nested multiplex PCR for detection of M. tu...
Mycobacterium tuberculosis resistance to anti-tuberculosis drugs is a major threat to global public ...
List of oligos used for the validation of DE genes and RT qPCR procedure. (DOCX 16 kb
Expected responses for knowledge assessments on molecular diagnostics; XpertMTB/RIF/GenotypeMTB/RIF ...
Technical appendix. A methods supplement providing technical details of the model. (PDF 197 kb
Lineage-specific SNPs for MTB Uganda family, MTB lineage 4 and MTB lineage 3. (DOCX 20 kb
IntroductionTuberculosis (TB) diagnosis still faces challenges with high proportion of bacteriologic...
Quality screening of sequencing data. This file details the quality control step of the study. (DOCX...
Detailed assay protocol. Table S1. DNA sequences for the oligonucleotides used in the 2-marker blood...
Figure S1. Distribution of sample numbers in three TB databases. Number of isolates with available D...
Supplementary Material 3: Table S4 List of primers used in this study for the detection of target ge...
Table S1. Investigating the presence of rpoB and 5KST diagnostic targets in M. tuberculosis complete...
Background: Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being u...
Background: Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being us...
Table S1. Taqman Gene Expression Assays used for qt-PCR. Table of Taqman primer probe sets for assay...
Comparison of conventional procedures with single-step & nested multiplex PCR for detection of M. tu...
Mycobacterium tuberculosis resistance to anti-tuberculosis drugs is a major threat to global public ...
List of oligos used for the validation of DE genes and RT qPCR procedure. (DOCX 16 kb
Expected responses for knowledge assessments on molecular diagnostics; XpertMTB/RIF/GenotypeMTB/RIF ...
Technical appendix. A methods supplement providing technical details of the model. (PDF 197 kb
Lineage-specific SNPs for MTB Uganda family, MTB lineage 4 and MTB lineage 3. (DOCX 20 kb
IntroductionTuberculosis (TB) diagnosis still faces challenges with high proportion of bacteriologic...
Quality screening of sequencing data. This file details the quality control step of the study. (DOCX...
Detailed assay protocol. Table S1. DNA sequences for the oligonucleotides used in the 2-marker blood...
Figure S1. Distribution of sample numbers in three TB databases. Number of isolates with available D...
Supplementary Material 3: Table S4 List of primers used in this study for the detection of target ge...