Phenotypic analysis of Δ<i>lptC</i> mutants.
- Mattia Benedet (2967495)
- Federica A. Falchi (2967498)
- Simone Puccio (358005)
- Cristiano Di Benedetto (2967501)
- Clelia Peano (67208)
- Alessandra Polissi (589441)
- Gianni Dehò (165480)
DOIAbstract
<p>Bacterial cultures grown in LB-glucose-chloramphenicol at 37°C were serially diluted 1:10 in microtiter wells and replica plated in LB agar plates all supplemented with glucose and chloramphenicol and, where indicated, bacitracin (50 μg/ml), novobiocin (10 μg/ml), rifampicin (2.5 μg/ml) or SDS-EDTA (0.5% and 0.25mM, respectively). MacConkey agar plates were supplemented with glucose and chloramphenicol. The plates were incubated overnight at 37°C (or 42°C, where indicated) or 3 d at 15°C, as indicated on top of the pictures. The serial dilutions are indicated on the bottom. Strains are indicated on the left of the panels by the relevant chromosomal mutant allele(s) followed by a slash and the plasmid encoded allele(s); the clones selecte...
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