Pterostilbene activated ATR/CHK1/p53 pathway to arrests the cell cycle in A549.

<p>(A) Hierarchical clustering of RPPA data from A549 cells treated with pterostilbene at the concentration of 2.5 μM and 5 μM for 48 hours in serum free media (n = 4 for each samples). Protein expression levels of A549 without pterostilbene treatment were used as a control. The data are presented in a matrix format: rows represent individual protein features (186 proteins), and columns represent individual samples. Each cell in the matrix represents the expression level of a protein feature in an individual cell sample (left). A total of 16 proteins showing significant differences with <i>P</i> < 0.05 by Student’s <i>t</i> test are shown in the right panel. (B) Western blotting results of A549 cells treated with for the molecules implicated in DNA damage responses and cell cycle regulations.