<p>(a) Silver stain and (b) densitometry to show proteins pulled down from CCR6DNP/TG-beads mixed with nuclear extract from THP-1 cells. Lane 1, BamH I fraction; Lane 2, BamH I fraction where pulldown was performed in the presence of a 40x excess of competitor (free CCR6DNP/TG). Arrow indicates the specific band sent for mass spectrometry analysis.</p
<p>(<b>A</b>) Description of the experiment in <i>C</i>. mPGRN-HA stably transfected HEK293 cells we...
<p>(A) Pull-down assays using MBP-PABPN1 deletion mutations as bait for WT GST-TTP. Each MBP-PABPN1 ...
a<p>Proteins were first ranked based on their frequency of detection in the Tmp1 affinity purificati...
<p>Mass spectrometry analysis identifies PARP-1 as the CCR6DNP binding protein.</p
<p><b>Panel A:</b> Pull down experiment of HeLa nuclear proteins incubated with the different RNA pr...
was resolved using SDS-PAGE and was either transferred to nitrocellulose for Far Western analysis (L...
<p>(<b>A</b>) Colloidal blue-stained gel for Ets-1-associated proteins from MDA-MB-231 nuclear extra...
<p>(A) Comparison of CD spectra of MBP-linker-MPR-TM and MBP-AAA-MPR-TM. (B) DLS measurement of 1 mg...
<p>(A) Scheme of the pulldown assay. The BG-ligand is immobilized on glutathione sepharose beads via...
<p>(A) Immunoprecipitation of GFP-tagged hPARP1 (141 kDa), hPARP2 (93 kDa), hPARP3 (87 kDa), hPARP9 ...
A 12% SDS-PAGE gel showing different elution fractions after crosslinking by formaldehyde and affini...
<p>(A) Pull-down assays to determine the interaction between MBP-PABPN1, (His)<sub>6</sub>-PAP-myc, ...
<p>(A) Activity assays of whole-cell, nuclear, and cytoplasmic cell fractions. n = 5; * p<0.05. (B) ...
<p><b>(A-B)</b> Survey spectra of BMP9-treated HUVEC focal adhesion preparations indicate the presen...
<p><b>A</b> Micrograph showing that Cot-DLAP locates to the cytoplasm and is enriched at growing cel...
<p>(<b>A</b>) Description of the experiment in <i>C</i>. mPGRN-HA stably transfected HEK293 cells we...
<p>(A) Pull-down assays using MBP-PABPN1 deletion mutations as bait for WT GST-TTP. Each MBP-PABPN1 ...
a<p>Proteins were first ranked based on their frequency of detection in the Tmp1 affinity purificati...
<p>Mass spectrometry analysis identifies PARP-1 as the CCR6DNP binding protein.</p
<p><b>Panel A:</b> Pull down experiment of HeLa nuclear proteins incubated with the different RNA pr...
was resolved using SDS-PAGE and was either transferred to nitrocellulose for Far Western analysis (L...
<p>(<b>A</b>) Colloidal blue-stained gel for Ets-1-associated proteins from MDA-MB-231 nuclear extra...
<p>(A) Comparison of CD spectra of MBP-linker-MPR-TM and MBP-AAA-MPR-TM. (B) DLS measurement of 1 mg...
<p>(A) Scheme of the pulldown assay. The BG-ligand is immobilized on glutathione sepharose beads via...
<p>(A) Immunoprecipitation of GFP-tagged hPARP1 (141 kDa), hPARP2 (93 kDa), hPARP3 (87 kDa), hPARP9 ...
A 12% SDS-PAGE gel showing different elution fractions after crosslinking by formaldehyde and affini...
<p>(A) Pull-down assays to determine the interaction between MBP-PABPN1, (His)<sub>6</sub>-PAP-myc, ...
<p>(A) Activity assays of whole-cell, nuclear, and cytoplasmic cell fractions. n = 5; * p<0.05. (B) ...
<p><b>(A-B)</b> Survey spectra of BMP9-treated HUVEC focal adhesion preparations indicate the presen...
<p><b>A</b> Micrograph showing that Cot-DLAP locates to the cytoplasm and is enriched at growing cel...
<p>(<b>A</b>) Description of the experiment in <i>C</i>. mPGRN-HA stably transfected HEK293 cells we...
<p>(A) Pull-down assays using MBP-PABPN1 deletion mutations as bait for WT GST-TTP. Each MBP-PABPN1 ...
a<p>Proteins were first ranked based on their frequency of detection in the Tmp1 affinity purificati...