Identification of the PABPN1 domains that interact with TTP.

<p>(A) Pull-down assays using MBP-PABPN1 deletion mutations as bait for WT GST-TTP. Each MBP-PABPN1 deletion mutant was individually immobilized on amylose resin and then incubated with GST-TTP. After extensive washes, the pulled-down complexes were separated by SDS-PAGE and visualized with Coomassie Blue (CB), or western blotted with anti-GST (WB). GST incubated with MBP-PABPN1@1-302 served as the control (lane 12). (B) Schematics of the PABPN1 constructs used in the pull-down assays. Heavy lines identify the included sequences. Plus signs indicate the relative amount of GST-TTP pulled down by the PABPN1 constructs, and a negative sign indicates that GST-TTP was not pulled down.