Protein VI is indirectly linked to loss of plasma membrane integrity.

<p>MoDCs were challenged with IC prepared from HAdV-C5 and HAdV-C5 harboring mutations in protein VI or protease. A) Effect on cell membrane integrity was determined by incubating MoDCs with HAdV-C5, Ad2ts1 and HAdV-C5 empty capsid or IC of each virus, respectively (n = 3) for 6 h, then with PI followed by flow cytometry. B) Dose-dependent loss of cell membrane integrity was assessed for IC-HAdV and IC-AdL40Q at 6 h (n = 5) as in B. Statistical significance (<i>p</i> values) were derived from two-way ANOVA with Bonferonni post test: * and ** correspond to p < 0.05 and p < 0.01, respectively.