Vesicular transport mediates PV-induced cytolysis.

<p>A9 cells were infected or not with MVMp (30 pfu/cell). Subsequent reinfections were prevented by neutralizing progeny particles released into the medium by addition of B7 antibodies. When indicated, protein functioning was altered by over-expression of specific mutant protein variants (Sar1K38M (dnSar1), GlnY438A (dnGln), Rab1N25S (dnRab1), Rab8T22N (dnRab8), Rab11N25S (dnRab11), Rdx<i>dl</i>[P], RdxY146F, MoeT547A (MoeA), respectively) transduced by rAAV 24 h prior to parvovirus infection. rMVM denotes transduction of A9 cells with a viral vector deficient for progeny virion particles production due to substitution of the transgene GFP for the structural gene region. Contr stands for non-infected cells. Treated cells were incubated with propidium iodide for 30 min at indicated times p.i. and detected in transmitted light and fluorescent microscopy. PI positive cells and total number of cells from 3 fields of at least three individual experiments (>200 cells) were counted using Image J and proportion of lysed cells calculated.