HspSP-ShM2 and HspWH7803 do not form hetero-oligomers at different concentrations of Triton™ X-100, different molar ratios nor under heat treatment.

<p>A and B) HspSP-ShM2 (dotted line) and HspS-WH7803 (dashed line) were incubated 30 min either alone or together (solid line) at a molar ratio of 1:1 at two different concentrations of Triton X-100™, (A) 0.23 mM and (B) 0.12 mM. Note that elution peaks of mixed sHSPs conditions for both detergent concentrations are equal as the ones of sHSPs alone. Protein elution volume was monitored by absorbance at 280 nm. The elution volumes of protein molar weight standards are shown above the first panel. C, D and E) Novex™ 4–20% Tris-Glycine native gels were used to compare the oligomeric profiles of (C) HspSP-ShM2 and HspS-WH7803 alone as controls for subsequent comparison (D) HspSP-ShM2 and HspS-WH7803 mixed together at different molar ratios and (E) HspS-ShM2 and HspS-WH7803 mixed together at an equimolar ratio, heat shocked at the indicated temperature, cooled down and migrated at room temperature (~25°C). The migration distances of protein molecular weight standards are shown between the panels C and D.