SEC and native gel analysis of HspSP-ShM2 and HspS-WH7803 oligomeric species.

<p>Each sHSP was analyzed at two different concentrations, 30 μM (solid line) and 60 μM (dotted line). A) HspSP-ShM2 first peak (*) elution volume is equivalent to ~ 600 kDa (32mer) and the second one (**) to ~ 200 kDa (dodecamer). B) HspS-WH7803 peak elution volume is equivalent to ~ 60 kDa (tetramer). C) Novex™ 4–20% Tris-Glycine native gels were used to compare the oligomeric profiles of HspSP-ShM2 and HspS-WH7803. The elution volumes of protein molecular weight standards are shown in the left panel.