I-BET-762 treatment suppresses AHR and inflammatory cell infiltration in RSV-induced exacerbation.

<p>BALB/c mice were sensitised i.p with OVA or PBS plus alum on day 0 and exposed to OVA aerosol on days 13–16. Some mice were inoculated with RSV on day 19 and treated with I-BET-762 or vehicle (hydroxyl-β cyclodextrin) i.v twice on day 23. Lung function and airway inflammation were assessed on day 24. (A) AHR lung assessments, (B) viral copies in total lung tissue and (C) total BALF cell counts. Differential microscopy counts for macrophages (D), neutrophils (E) eosinophils (F), and lymphocyte (G) performed by light microscopy. n = 6–8 mice per group, presented as mean ± SEM. *Designates significant differences to PBS-treated controls (*P<0.05, **P<0.01, ***P<0.001), ^#Designates significant differences from OVA/RSV/I-BET-762-treated mice (^#P<0.05, ^##P<0.01).