Localization by fluorescence microscopy of the GFP-ERF and GFP-DOF fusion proteins in root cells.

<p>A nuclear localization was observed in root cells near the root tip of transgenic Arabidopsis plants constitutively expressing the GFP-AhERF-VII (panels E and F) or the GFP-AhDOF-AI (panels G and H) fusion proteins. The results were compared with those obtained from control transgenic Arabidopsis plants constitutively expressing the 35S:: GFP-GUS (panels A and B) or the H2B:: GFP fusion proteins (panels C and D). The latter overexpress a histone 2B fused with GFP (H2B::GFP), which is a commonly employed nuclear marker.