<i>Arabidopsis</i> UAP56 localises to the nucleus in protoplasts and root cells.

<p>Tobacco BY2 cell protoplasts were transformed with constructs driving the expression of the indicated GFP fusion proteins and GFP fluorescence was visualised by CLSM (<b>A,B</b>; size bar: 10 µm). GFP fluorescence of a nuclear HMGB protein and overlay with the corresponding bright field image (<b>A</b>). GFP fluorescence of a UAP56-GFP fusion (<b>B</b>). Analysis of <i>Arabidopsis</i> Col-0 root tip cells by immunofluorescence microscopy using different antibodies and DAPI staining (<b>E</b>–<b>H</b>, size bar: 5 µm). Immunostaining of fibrillarin and UAP56, as well as visualisation of the DNA by DAPI staining of the same nucleus (<b>C</b>–<b>E</b>). A merge of the three images is shown in (<b>F</b>) and examples of brightly DAPI-stained heterochromatic chromocenters are indicated by arrows. Immunostaining of RNAPII and UAP56 of the same nucleus (<b>G,H</b>).