Effect of ALF-186 treatment on retinal protein and mRNA expression of Bcl-2.
- Felix Ulbrich (678720)
- Kai B. Kaufmann (826015)
- Alexander Meske (3233784)
- Wolf A. Lagrèze (157418)
- Michael Augustynik (3233790)
- Hartmut Buerkle (131054)
- Carlos C. Ramao (3233787)
- Julia Biermann (131071)
- Ulrich Goebel (131076)
Publication date
October 2016
DOIAbstract
<p>(A) Fold induction of Bcl-2 mRNA after IRI+ALF-186 and p38 inhibition in ischemic retinal tissue compared to GAPDH in relation to the corresponding non-ischemic retinae analyzed by RT-PCR (n = 8; data are mean±SD; IRI vs. IRI+ALF-186, *** = p<0.001 and IRI+ALF-186 vs. SB203580+IRI+ALF-186, *** = p<0.001). (E) Representative western blot image showing no change of Bcl-2 due to IRI (lane 2) but its strong induction (lane 4) compared to β-Actin after ALF-186 treatment. This effect was abrogated by p38 inhibition using SB203580 (lane 6). (F) Densitometric analysis of n = 8 western blots for Bax expression (data are mean±SD; IRI vs. IRI+ALF-186, ** = p<0.01 and IRI+ALF-186 vs. SB203580+IRI+ ALF-186, * = p<0.05).</p
Add to ORKG