S711E disrupts trafficking to lysosomes.

<p>SN56 cells were transfected with plasmids expressing wild type APP, S711E, or S711A. Concomitantly, plasmids expressing LAMP1-mRFP or Rab5-mRFP and GalT-CFP were also transfected. <b>a)</b> Representative images depicting trafficking of APP S711A or S711E to lysosomes (LAMP1-mRFP) after photo-activation in GalT-CFP labeled compartments. <b>b)</b> Representative images showing the delivery of APP S711A or S711E to early endosomes after photo-activation. The edge of the cell is defined by the white line, and was drawn based on the white light images. Scale bars represent 5μm for all images. Triangles with circles denote photo-activation sites at time 0. Triangles alone point to colocalized pixels. <b>c)</b> The percentage of APP colocalized with either LAMP1 or Rab5 was quantified with Imaris. Error bars denote SEM. * = p<0.05.