Tyrosine disrupts internalization into early endosomes.

<p>SN56 cells were transfected with βAPP-CFP (with or without tyrosine mutations) and Rab5-mRFP. The HA-tag on our βAPP-CFP construct was fluorescently labeled using an anti HA-Zenon conjugate. <b>a-d)</b> Representative images of βAPP-CFP, bearing one of the tyrosine mutations, internalized into Rab5-mRFP compartments after 15-minutes. The edge of the cell is shown by the white border, and was drawn based on white-light images. Triangles point to colocalized pixels. Scale bars represent 5μm. <b>e)</b> The percentage of APP co-localized with Rab5 was quantified using Imaris and graphed (* = p<0.05, error bars represent SEM).