The effect of immune modulation on RAW 264.7 COX-2 expression when infected with Bp82 after 6 hours of infection.

<p>Cells were pretreated with 100 μM TA or 0.01% DMSO for 30 minutes along with an uninfected and untreated control. COX-2 levels were determined flow cytometry using the Mean Channel Fluorescence (MCF) over 5,000 events from intact non-aggregated cells (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005065#pntd.0005065.s002" target="_blank">S1 Fig</a>). Significance was determined by comparing to the vehicle control, where TA treatment was able to suppress COX-2 expression significantly. Data is representative of three biological replicates run in duplicate. Significance was determined via 2-way ANOVA with Bonferroni’s post-hoc test for significance. Error indicates SEM. **p<0.01, ****p<0.0001.