Control of IL-8 Secretion by Analogue 6 in KLK5 Simulated N-tert Skin Cells.

<p>Changes in IL-8 level in the cell medium was monitored 6, 24 and 48 hours after identical cell populations were stimulated with fresh medium (Medium Only), or KLK5 (KLK5 Only, f/c 300 nM), or KLK5 + Analogue 6 (KLK5 + Analogue 6), or Analogue 6 (Analogue 6 Only, f/c 1515 nM), or 0.2 mM MES (Medium + 0.2 mM MES). All substances were diluted with cell culture medium. Control samples including the medium with MES were used to ensure that MES which was in the KLK5 protein stock solution did not cause IL-8 level to change. Basal IL-8 level prior to stimulation (0hr pre-treatment) as well as for unstimulated cells 48hrs after (48hr untreated) were also determined to serve as quality controls. A coefficient variation of around 11% and 6% for 0hr pre-treatment and 48hr untreated respectively indicates the status of cell populations in each well was homogenous throughout the experiment. Error bars are standard deviations of IL-8 readings from different wells of cells. N.S. Not Statistically Significant; * p<0.05; **p<0.01.