Depletion of BRD2 or BRD4 activates KSHV lytic cycle.

<p><b>(A)</b> Western blot of BCBL1 cells transduced with shControl (shCtrl) or shBRD4, probed for BRD4 (top) or Actin (bottom). <b>(B)</b> RT-qPCR for PAN and ORF50 relative to actin in BCBL1 cells transduced with shCtrl or shBRD4. <b>(C)</b> Western blot of BCBL1 cells transduced with shCtrl or shBRD2 probed for BRD2 (top) or Actin (bottom). <b>(D)</b> RT-qPCR for PAN and ORF50 relative to actin in BCBL1 cells transduced with shCtrl or shBRD2. <b>(E)</b> PFGE analysis of BCBL1 cells transduced with shCtrl, shBRD2 (in duplicate), or shBDR4 (in duplicate) as indicated, and assayed by Southern blot with KSHV genome probe.