NLRX1 is required for optimal KSHV reactivation in BCBL-1 cells.

<p>BCBL-1 cells were transfected with NS or NLRX1 siRNA for 48 hours and then treated with TPA and sodium butyrate for various time points. (A) KSHV genome copy number in reactivated BCBL-1 cells. (B) KSHV genome copy number in the supernatants of reactivated BCBL-1 cells. (C) qRT-PCR of NLRX1 in reactivated BCBL-1 cells. (D) qRT-PCR of ORF57 in reactivated BCBL-1 cells. (E) qRT-PCR of K8.1 in reactivated BCBL-1 cells. (F) qRT-PCR of ORF36 in reactivated BCBL-1 cells. Data are presented as mean ± s.d. from at least three independent experiments. * indicates p<0.05. ** indicates p<0.01 by Student’s t-test.