Romidepsin, Panobinostat, and PTC-209 induce KSHV genome replication and release of infectious virions.

(A) qPCR analysis for cell-associated KSHV genome copies was performed on BCBL-1 cells treated with DMSO or 1 μM Romidepsin or Panobinostat for five days. Naïve Vero cells were infected with supernatants collected from BCBL-1 cells treated as in (A), and KSHV genome copies in the infected cells were determined by qPCR (B), and KSHV lytic transcript levels were assayed by RT-qPCR (C). Lytic transcript levels were normalized to GAPDH, and the fold difference between DMSO and compound treated cells was calculated. (D) qPCR analysis for cell-associated KSHV genome copies was performed on BCBL-1 cells treated with DMSO or 10 μM PTC-209 for seven days. Naïve Vero cells were infected with supernatants collected from BCBL-1 cells treated as in (D), and KSHV genome copies in the infected cells were determined by qPCR (E), and KSHV lytic transcript levels were assayed by RT-qPCR (F). Lytic transcript levels were normalized to GAPDH, and the fold difference between DMSO and PTC-209 treated cells was calculated.