Conserved sequences around Q170 residue in olNbs1 and distribution of Q170/H170 alleles in wild medaka populations.

<p>(A) Schematic drawing of NBS1 protein domain structure (top) and alignment of amino acid sequences around Q170 residue of olNbs1 (bottom) are shown. FHA domain and two BRCT domains are present in the N-terminal region of NBS1 and Q170 locates in the flange part between BRCT1 and BRCT2 domains in olNbs1. Twenty-one amino acids for Hs, <i>Homo sapiens</i> (from E175 to S195 of ENST00000265433.7); Mm, <i>Mus musculus</i> (from E175 to S195 of ENSMUST00000029879.14); Ol, <i>Oryzias latipes</i> (Hd-rR, from E160 to S180 of ENSORLG00000009450.1); stickleback (from D173 to S193 of ENSGACT00000016251); platyfish (from E161 to S181 of ENSXMAT00000007002); fugu (from E163 to R183 of ENSTRUT00000005862); tetraodon (from E174 to R194 of ENSTNIT00000021752), zebrafish (from A162 to R182 of ENSDART00000058974) are aligned from the ENSEMBL database and the consensus amino acids are indicated. Hydrophobic residues are highlighted in gray boxes, and amino acid residues with a long straight side chain (≥ 3 carbons) are highlighted in black boxes. (B) Distribution of olNbs1 (Q170) and olNbs1 (H170) alleles in the wild medaka populations. Parenthesized numbers refer to the ID numbers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170006#pone.0170006.t001" target="_blank">Table 1</a>. Open circles represent the collection sites where homozygotes of the olNbs1 (Q170) allele were found. Filled circles with numbers represent the collection sites where homozygotes of the olNbs1 (H170) alleles were found. Asterisks indicate the collection sites where heterozygotes of the olNbs1 (Q170) allele and the olNbs1 (H170) allele were found.