Reducing DAF-2/IIS signaling mitigates the secretory block in <i>ire-1</i> mutant animals.

<p>A–D. Fluorescent micrographs of animals transgenically expressing a translational DMA-1::GFP fusion [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006579#pgen.1006579.ref012" target="_blank">12</a>] in the genotypes indicated. Anterior is to the left in all panels and ventral down. Arrowheads indicate the PVD cell body and scale bars 20 μm. E. Quantification of mean fluorescent intensity of the PVD cell body in different genetic backgrounds. F. Quantification of defects in PVD in animals in the indicated genotypes. G. Quantification of branch number in <i>daf-2(e1370)</i>, <i>ire-1(ok799)</i>, <i>dma-1(tm5159)</i> single-, double- and triple-mutant animals. Data are represented as mean ± SEM. Statistical comparisons were performed using one-way ANOVA with the Tukey correction, and statistical significance is indicated (*p = 0.05, **p = 0.01, ***p = 0.001, ****p = 0.0001; ns, not significant [p > 0.05]). n = 20 wild-type controls (1295 dendritic branches), n = 20 <i>daf-2(e1370)</i>mutant animals (1046 dendritic branches), n = 20 <i>ire-1(ok799)</i> mutant animals (489 dendritic branches), n = 20 <i>dma-1(tm5159)</i> mutant animals (381 dendritic branches), n = 20 <i>ire-1(ok799); daf-2(e1370)</i> mutant animals (1100 dendritic branches), n = 19 <i>dma-1(tm5159); ire-1(ok799); daf-2(e1370)</i> mutant animals (470 dendritic branches). Data for wild-type control and <i>ire-1(ok799)</i> animals are identical to those in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006579#pgen.1006579.g001" target="_blank">Fig 1</a> and are shown for comparison only.