Related to Fig 1- The available PRDX-2::GFP transgenic line (CTD1051.3) does not suit to our study.

(A) Low magnification fluorescent image of untreated animals from the CTD1051.3 line showing that transgenics express aggregates of PRDX-2::GFP, a hallmark of overexpression of the fusion protein. (B,C) Low magnification images of animals treated in flat bottom wells imaged after 24h of treatment in the potent oxidative stress inducing agent tBOOH. In the wild-type control well (A), almost all animals are all dead in 2mM tBOOH, appearing as rods, while CTD1051.3 transgenics survive a 25X higher dose of the drug, indicating their much stronger resistance to oxidative stress. (D) Representative image of an animal of the PRDX-2::GFP knock-in line; a wild-type control imaged with the same settings is shown (E), delineated by a dotted line. Bar, 100μm. (F-H) Confocal projections showing the tail region (dotted contours) of a PRDX-2::GFP transgenic animal stained with the lipophilic orange-red dye DiI (see https://www.wormatlas.org/EMmethods/DiIDiO.htm), imaged in green (F) and red channels (G). The overlay (H) shows that PRDX-2::GFP tail neurons are stained by the dye, establishing their identity as phasmid sensory neurons (PHA/PHB). Bar, 10μm. (TIF)