<p>(A) Activity of HCHT on MU-NAG<sub>2</sub> substrate as a function of substrate concentration. (B) NAG<sub>2</sub> inhibition of HCHT on MU-NAG<sub>2</sub> substrate measured at 3 different substrate concentrations—0.5 μM, 5 μM or 50 μM. (C) NAG<sub>2</sub> inhibition of HCHT on <sup>14</sup>C-CNW substrate (1.0 g/L). <i>v</i><sub>i</sub> and <i>v</i><sub>i = 0</sub> stand for the rates measured in the presence and absence of inhibitor, respectively. Error bars show standard deviations and are from three independent experiments.</p
<p>Values are means of two to five experiments ± standard deviation.</p>†<p>ND, not determined.</p><...
<p>The rate of substrate Suc-LLVY-AMC hydrolysis (a release of AMC molecule) with control and inhibi...
<p>Cells were exposed to a concentration range of 5-FU, Sal and their combination for 48 h.</p><p>Va...
<p>NI–no inhibition observed; K<sub>m</sub> values relate to the substrate used for analysis,</p><p>...
<p>Bars indicate the difference between the percentage inhibition observed using 0.11 mM GAL as subs...
<p>The concentration of the inhibitor was kept at 100 µM and the readings were taken under two subst...
<p>Reciprocal initial velocities (Fig. 2) for two substrate conentrations were replotted against inh...
<p>Percentage residual activity was determined at various inhibitor concentrations using the followi...
<p>A) CGP-74514A, B) P2, C) P6, D) P7, E) P8 compared to no inhibitor (gray squares) or no enzyme (g...
<p>The <i>k</i><sub>inact</sub> and <i>K</i><sub>I</sub> values for the covalent inhibitors were obt...
[a]Assays were performed in duplicate (errors were less than 5%) with CENTA as reporter substrate (1...
<p>Note: The concentrations of CHL was 5 µM and that for the other specific inhibitors was 1 µM. The...
<p>(A) Correlation analysis between inhibition of OCT2-mediated metformin and inhibition of OCT2-med...
<p>NT<i>Hi</i> Nuc was incubated with 2 μM FRET substrate and increasing concentrations of inhibitor...
<p>(<b>A</b>) Inhibition by Mg·AMP with Mg·ADP as variable substrate. Measurements were assayed at f...
<p>Values are means of two to five experiments ± standard deviation.</p>†<p>ND, not determined.</p><...
<p>The rate of substrate Suc-LLVY-AMC hydrolysis (a release of AMC molecule) with control and inhibi...
<p>Cells were exposed to a concentration range of 5-FU, Sal and their combination for 48 h.</p><p>Va...
<p>NI–no inhibition observed; K<sub>m</sub> values relate to the substrate used for analysis,</p><p>...
<p>Bars indicate the difference between the percentage inhibition observed using 0.11 mM GAL as subs...
<p>The concentration of the inhibitor was kept at 100 µM and the readings were taken under two subst...
<p>Reciprocal initial velocities (Fig. 2) for two substrate conentrations were replotted against inh...
<p>Percentage residual activity was determined at various inhibitor concentrations using the followi...
<p>A) CGP-74514A, B) P2, C) P6, D) P7, E) P8 compared to no inhibitor (gray squares) or no enzyme (g...
<p>The <i>k</i><sub>inact</sub> and <i>K</i><sub>I</sub> values for the covalent inhibitors were obt...
[a]Assays were performed in duplicate (errors were less than 5%) with CENTA as reporter substrate (1...
<p>Note: The concentrations of CHL was 5 µM and that for the other specific inhibitors was 1 µM. The...
<p>(A) Correlation analysis between inhibition of OCT2-mediated metformin and inhibition of OCT2-med...
<p>NT<i>Hi</i> Nuc was incubated with 2 μM FRET substrate and increasing concentrations of inhibitor...
<p>(<b>A</b>) Inhibition by Mg·AMP with Mg·ADP as variable substrate. Measurements were assayed at f...
<p>Values are means of two to five experiments ± standard deviation.</p>†<p>ND, not determined.</p><...
<p>The rate of substrate Suc-LLVY-AMC hydrolysis (a release of AMC molecule) with control and inhibi...
<p>Cells were exposed to a concentration range of 5-FU, Sal and their combination for 48 h.</p><p>Va...