Effect of prolonged room-temperature incubation on the antigenicity of HIV-1 Env-NDs.

<p>(A,B) Binding of the indicated ligands to the HIV-1_JR-FL Env(-)Δ808-NDs that had been incubated at room temperature for a week is compared to that of the same ligands to glutaraldehyde-treated and untreated HIV-1_JR-FL Env(-)Δ808 glycoprotein on the surface of expressing cells. Shading of the ligands is described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0170672#pone.0170672.g006" target="_blank">Fig 6</a>. (<b>C</b>) The binding of the indicated ligands to the HIV-1_JR-FL Env(-)Δ808 E168K-NDs that had been incubated at room temperature for one week is shown. The E168K change allows the HIV-1_JR-FL Env to be recognized by the V2 quaternary-dependent antibody PG9 (101). * = p < 0.05, unpaired t-test. ND–Not determined.