Amino acid sequence comparison of GalNAc-binding <i>C</i>. <i>perfringens</i> CBM32s.

<p>Sequence alignment of the three <i>Cp</i>GH31 CBM32 modules with other functionally characterized CBM32 modules from the following family 33, family 84, and family 89 glycoside hydrolases with specificity for galacto- or gluco-configured sugars: <i>Cp</i>GH33 CBM32, galacto-configured sugar specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref021" target="_blank">21</a>]; <i>Cp</i>GH84A CBM32-1, galacto-configured sugar specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref024" target="_blank">24</a>]; <i>Cp</i>GH84A CBM32-2, GlcNAc specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref023" target="_blank">23</a>]; <i>Cp</i>GH84C CBM32, galacto-configured sugar specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref022" target="_blank">22</a>]; <i>Cp</i>GH89 CBM32-3 and CBM32-4, GlcNAc-α-1,4-Gal specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref020" target="_blank">20</a>]; <i>Cp</i>GH89 CBM32-5, galacto-configured sugar specificity [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref020" target="_blank">20</a>]. Positions comprising conserved amino acid residues are identified by white single-letter code and highlighted in red while positions displaying amino acid residues of similar physicochemical properties are identified by red-single letter code. Sugar-coordinating amino acid residues in each CBM32 seequence are identified by black boxes. The sequence alignment was created using CLUSTAL OMEGA [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref045" target="_blank">45</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref046" target="_blank">46</a>] and displayed using ESPript [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171606#pone.0171606.ref047" target="_blank">47</a>].