Sequence alignment of ginsenoside-transforming BglPC28 and Bgp1 or structure-determined glycoside hydrolase family 3 enzymes.

<p>The sequence alignment was created using ClustalW at the EBI-server using default settings and visualized with Jalview (<a href="http://www.ebi.ac.uk/Vmichele/jalview/" target="_blank">http://www.ebi.ac.uk/Vmichele/jalview/</a>). Regions of identity or high similarity among sequences are shown as black or gray columns, respectively. The conserved active site residues (general acid/base and nucleophile residues) were marked by asterisk. The PA14 domains of <i>S.ven</i> and <i>K.mar</i> are boxed. Genbank IDs of the glycoside hydrolases family 3 are as follows, <i>Pseudonocardia</i> sp. Gsoil 1536 β-glucosidase [<i>BglPC28</i> (This study)], JX960416; <i>Microbacterium esteraromaticum</i> KACC 16318 β-glucosidase (<i>Bgp1</i>), AEX88466; <i>Streptomyces venezuelae</i> β-glycosidase (<i>S.ven</i>), AAC68679; <i>Thermotoganeapolitana</i>DSM 4359^T β-glycosidase (<i>T.nea</i>), ABI29899; <i>Kluyveromycesmarxianus</i> β-glucosidase (<i>K.mar</i>), ACY95404.