<p>(A) Immunofluorescence assay using purified anti-TcHMGB antibody and parasites at different stages of the <i>T</i>. <i>cruzi</i> life cycle: Epimastigotes, Free Amastigotes released from infected Vero cells and Trypomastigotes treated (+) and non-treated (-) with deacetylase inhibitors (iDAC) Trichostatin A, Sodium Butyrate and Nicotinamide. Anti-rabbit IgG conjugated to fluorescein was used as a secondary antibody. Nuclei and kinetoplasts were labeled with DAPI. Bars = 2 μm. (B) Slot western blot assay. Culture supernatants in FCS-free media were treated (+) and non-treated (-) with deacetylase inhibitors (iDAC) and blotted onto nitrocellulose membranes. Epimastigotes: E, Infected Vero cells: I C, Trypomastigotes: T, and un-infected Ver...
<p>A) Fluorescence microscopy of <i>T. cruzi</i> epimastigotes in exponential growth phase, treated ...
Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote...
Background Trypanosoma cruzi is a parasitic protist that causes Chagas disease, which is prevalent i...
<p>TcCat immunolocalization (<i>green</i>) in <i>T. cruzi</i> trypomastigotes (<b>A</b>), epimastigo...
<p>A) TcADKn localization in epimastigotes of <i>T. cruzi</i> along the growth curve. Parasites were...
<p>Live metacyclic trypomastigotes were incubated for 1 h with mAb 3F6 (A) or polyclonal anti-J18 an...
<p><b>A–C:</b> Indirect immunofluorescence was performed on unpermeabilized trypomastigotes using an...
<p>Lysates of <i>T. cruzi</i> trypomastigotes were treated with CIAP (<b>A</b>) or glycosidases (<b>...
<p>Indirect immunofluorescence analysis using anti-TcSUMO antibody of epimastigotes (Epi; a–h), meta...
<p>(A) Immunofluorescence of infective metacyclic trypomastigotes showing staining with anti-TS-SAPA...
<p>Cardiomyocytes pre-treated or not with different cyclodextrins were washed and challenged with <i...
<p>(A) Increasing amounts of the purified recombinant protein SAP-CE or GST were added to 96-well pl...
<p>A) Fluorescence assay of trypomastigotes incubated with rGal–1 (25 μg/ml) for 1 h, followed by in...
<p><b>A.</b> Western blot of total protein extracts from CL Brener trypomastigotes (T), epimastigote...
<p>A) Samples of <i>T</i>. <i>cruzi</i> (CL strain) (1 x 10<sup>7</sup> cells/slot) and <i>T</i>. <i...
<p>A) Fluorescence microscopy of <i>T. cruzi</i> epimastigotes in exponential growth phase, treated ...
Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote...
Background Trypanosoma cruzi is a parasitic protist that causes Chagas disease, which is prevalent i...
<p>TcCat immunolocalization (<i>green</i>) in <i>T. cruzi</i> trypomastigotes (<b>A</b>), epimastigo...
<p>A) TcADKn localization in epimastigotes of <i>T. cruzi</i> along the growth curve. Parasites were...
<p>Live metacyclic trypomastigotes were incubated for 1 h with mAb 3F6 (A) or polyclonal anti-J18 an...
<p><b>A–C:</b> Indirect immunofluorescence was performed on unpermeabilized trypomastigotes using an...
<p>Lysates of <i>T. cruzi</i> trypomastigotes were treated with CIAP (<b>A</b>) or glycosidases (<b>...
<p>Indirect immunofluorescence analysis using anti-TcSUMO antibody of epimastigotes (Epi; a–h), meta...
<p>(A) Immunofluorescence of infective metacyclic trypomastigotes showing staining with anti-TS-SAPA...
<p>Cardiomyocytes pre-treated or not with different cyclodextrins were washed and challenged with <i...
<p>(A) Increasing amounts of the purified recombinant protein SAP-CE or GST were added to 96-well pl...
<p>A) Fluorescence assay of trypomastigotes incubated with rGal–1 (25 μg/ml) for 1 h, followed by in...
<p><b>A.</b> Western blot of total protein extracts from CL Brener trypomastigotes (T), epimastigote...
<p>A) Samples of <i>T</i>. <i>cruzi</i> (CL strain) (1 x 10<sup>7</sup> cells/slot) and <i>T</i>. <i...
<p>A) Fluorescence microscopy of <i>T. cruzi</i> epimastigotes in exponential growth phase, treated ...
Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote...
Background Trypanosoma cruzi is a parasitic protist that causes Chagas disease, which is prevalent i...