Expression and purification of recombinant Rv2190c protein as determined by SDS-PAGE electrophoresis. The gel was subjected to electrophoresis followed by Coomassie blue staining. Lane M, protein molecular weight marker. Lane 1, E. coli lysates engineered before isopropylthiogalactopyranoside (IPTG) induction. Lane 2, E. coli lysates engineered after induction with IPTG. Lane 3, the purified recombinant Rv2190c protein. (TIF 39 kb
Standard curve of BCG Pasteur Aeras used to convert TTP to CFU. A linear regression analysis was car...
Figure S1. Confirmation of ARL and AR insert in pRSF:Duet plasmid by double restriction enzyme diges...
Table S6. Data set from cytokine response profiling of GIT for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2 (p...
Fragment sizes of restriction-enzyme-digested recombinant plasmid pVAX1-rv2190c. 1. PCR amplificatio...
Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (...
Table S2. Data set of whole cell ELISA of L. plantarum attached with ACERL for a four-day period. (X...
Additional file 2: Figure S2. Comparison of antibody recognition of P. pastoris rPstS-1 and E. coli ...
Table S1. Data set of L. plantarum Pa21 carrying ACERL antigen growth profiles. (XLSX 25 kb
Table S3. Data set from ELISA for time-course of the serum total IgG antibody response against Lp AC...
Additional file 2: Figure S2. Gating procedure for monitoring the expression of CXCR3 + CCR6 on IFN-...
Additional file 2: Figure S1. Titration of peptide dose for in vitro stimulation. Mice were immunize...
Table S7. Data set from cytokine response profiling of re-stimulated splenocytes with ACERL antigen ...
Table S4. Data set from cytokine response profiling of spleen for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2...
Table S5. Data set from cytokine response profiling of lung for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2 (...
Additional file 1: Table S1. Description of lipidated and free peptides in the vaccine. Table shows ...
Standard curve of BCG Pasteur Aeras used to convert TTP to CFU. A linear regression analysis was car...
Figure S1. Confirmation of ARL and AR insert in pRSF:Duet plasmid by double restriction enzyme diges...
Table S6. Data set from cytokine response profiling of GIT for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2 (p...
Fragment sizes of restriction-enzyme-digested recombinant plasmid pVAX1-rv2190c. 1. PCR amplificatio...
Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (...
Table S2. Data set of whole cell ELISA of L. plantarum attached with ACERL for a four-day period. (X...
Additional file 2: Figure S2. Comparison of antibody recognition of P. pastoris rPstS-1 and E. coli ...
Table S1. Data set of L. plantarum Pa21 carrying ACERL antigen growth profiles. (XLSX 25 kb
Table S3. Data set from ELISA for time-course of the serum total IgG antibody response against Lp AC...
Additional file 2: Figure S2. Gating procedure for monitoring the expression of CXCR3 + CCR6 on IFN-...
Additional file 2: Figure S1. Titration of peptide dose for in vitro stimulation. Mice were immunize...
Table S7. Data set from cytokine response profiling of re-stimulated splenocytes with ACERL antigen ...
Table S4. Data set from cytokine response profiling of spleen for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2...
Table S5. Data set from cytokine response profiling of lung for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2 (...
Additional file 1: Table S1. Description of lipidated and free peptides in the vaccine. Table shows ...
Standard curve of BCG Pasteur Aeras used to convert TTP to CFU. A linear regression analysis was car...
Figure S1. Confirmation of ARL and AR insert in pRSF:Duet plasmid by double restriction enzyme diges...
Table S6. Data set from cytokine response profiling of GIT for IFN-Îł (pg/ml), IL-4 (pg/ml), IL-2 (p...