MOESM2 of Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Additional file 2: Figure S2. Comparison of antibody recognition of P. pastoris rPstS-1 and E. coli expressed His-tag-rPstS-1. Western blotting performed of rPstS-1 produced in P. pastoris (lanes 1, 2 and 3, using 2.0, 1.0 and 0.5 μg of rPstS-1, respectively) or E. coli (lanes 4, 5 and 6, using 2.0, 1.0 and 0.5 μg of rPstS-1, respectively), incubated with A) a rabbit anti-Mycobacterium tuberculosis-PstS-1 polyclonal antibody and B) a human serum sample from patient clinically confirmed active TB. For each blot, molecular mass markers (kDa) are indicated on the right