A decrease in CD44+ cells in response to hypoxia.

<p>(A) MU020 and MU039 cells were incubated for 24 hours in a hypoxic chamber, followed by immunophenotyping for CD44 and CD133 expression. (B) Changes in surface protein expression for cells grown in hypoxia were quantified as the difference from the proportion of cells expressing the marker in control (atmospheric oxygen) cells. (C) Representative FACS analysis of MU020. PDGCs were treated with a single 6Gy dose of radiation followed daily treatment with 50μM TMZ or combination therapy. Five days later cells were immunophenotyped for CD44 and CD133 expression. (D) Effect of GBM therapy on expression of CD44 and CD133. Changes in surface expression was quantified as the difference from control treated (DMSO) cells. Error bars represent SEM of 4 distinct PDGCs.