Hypoxia-induced changes in cell morphology and staining patterns consistent with EMT.

<p><b>A</b>; phase-contrast images of Ca1, H357 and LuC4 cells grown under normoxic and 0.2% hypoxic culture conditions for 21 days (insets: higher magnification of EMT-like cells). <b>B</b>; cytometric analyses indicating a shift in staining for CD44 (y axis) and ESA (x axis) of cells resulting from hypoxia. <b>C</b>; analysis of the increases in EMT resulting from hypoxia. <b>D</b>; growth of the Ca1 cell line for 21 days with 1 mM DMOG results in morphological changes (top), corresponding changes in CD44 and ESA staining (middle), stabilisation HIF-1α but little change in HIF-2α (bottom left), and a increase in the EMT fraction (bottom right). <b>E</b>; time dependant decrease in the size of hypoxia-induced EMT fractions after return to normoxic conditions.