Additional file 1: Figure S1. of Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines

Effect of FABP7 overexpression on the 786-O cell line. 786-O cells were cultured for two days in RPMI 1640 medium containing 10% FBS, 5 mg/L blasticidin S HCl, 0.3 g/L G418, and 1 mg/L doxycycline hyclate. a, Western blot analysis of FABP7 expression by cells transfected with the FABP7 vector or control (lacZ) vector. b, Real-time PCR analysis of FABP7 expression of cells transfected with the FABP7 vector or lacZ vector. c-d, The 786-O transfectants were cultured in RPMI-1640 medium containing 10% FBS or 1% FBS with 5 mg/L blasticidin S HCl, 0.3 g/L G418, and 1 mg/L doxycycline hyclate and subjected to cell proliferation and migration assays. c, The doubling times of cell transfected with the FABP7- or the lacZ-expression vector were determined using an MTS assay. The data represent the average and standard deviation (error bars) of five experiments. d, The migration of 786-O cells transfected with the FABP7- or lacZ-expression vector was determined using a wound-healing assay. The data represent the average and standard deviation (error bars) of four experiments. (TIFF 2702 kb