Effects of compound 2 on histopathology of liver fibrosis.

<p>Forty-eight hours after hydrodynamics-based injection of pPK9a, the mice were sacrificed and the liver sections were subjected to Masson’s trichrome staining (A) and immunohistochemistry (B). (A) Detection of ECM and collagen by Masson’s trichrome staining. The cytoplasm is red and collagen fiber is blue-green. The collagen signals were indicated by arrows. (B) Detection of α-SMA by immunohistochemistry. Dark brown granules represent α-SMA signals stained by α-SMA-specific antibody and were indicated by arrows. Representative liver sections of collagen (A) or α-SMA (B) from experimental I-VI groups: (I) Ringer’s solution + pPK9a + ZnSO₄ 48 h, (II) Ringer’s solution + pPK9a + ZnSO₄ + 30 μg/kg/day compound 2, (Ⅲ) Ringer’s solution + pPK9a + ZnSO₄ +50 μg/kg/day compound 2, and (IV) Ringer’s solution + pPK9a + ZnSO₄ + 300 μg/kg/day compound 2. Original magnification: I-IV, 200x.