Effect of MC1568 treatment on fibrogenesis in a CCl₄ induced fibrosis mouse model.

<p>Mice were CCl₄ treated twice a week for a total period of 4 weeks. After the second week, mice also received intraperitoneal injections with MC1568 (50 mg/kg) every two days for two more weeks. The day after the final CCl₄ injection, mice were sacrificed and liver tissue was extracted for analysis. (A) Sirius Red staining was performed to visualize deposited collagens. Image J software was used for quantification of the red surface area. Scale bar = 100 µm. (B) Serum levels of ALT and AST. (C) HDAC activity was measured in protein lysates of livers at the end of treatments using HDAC-Glo. (D) A mathematical correlation between the HDAC activity and the red stained area, after Sirius staining was determined using Pearson correlation method. The a and b refer to HDAC-activity values of the corresponding liver lysates of the images given for CCl₄ + MC1568 in A).