Competitive fitness measurements of reconstructed genotypes.

<p>A) The resolution of our assay was assessed by performing 64 competitions of wild-type spores from the SGA process carrying identical marked alleles from a mosaic of BY4741/BY4742 parental background and are assayed using our high-throughput fitness assay (grey curve). We indicate the measured fitness effects of strains carrying mad3Δ, bub1Δ, or the single-copy protein at the <i>BUB1</i> locus as red and green vertical bars. The two bars for each genotype represent measurements of relative selection coefficient to the wild-type control from replicate strains carrying swapped fluorescent proteins. B) Heat map clustering of all the competitive fitness assays using Euclidean distance and average linkage of the selection coefficients. The transpose off-diagonals are biological replicates with strains carrying swapped fluorescent proteins. Three major fitness classes are observed, which correspond to genotypes that have fitness comparable to mad3Δ cells, bub1Δ cells, or to the wild-type (as indicated by the mean selection coefficient measured for the strains present in each cluster). Grey cells in the heat map indicate competitions that were not performed.