Maternal HFD increases pro-fibrogenic response in offspring liver following MCD diets.

<p><b>(A)</b> Photomicrographs of Picro-Sirius red stain showing peri-cellular fibrosis. <b>(B)</b> Real-time PCR quantification of the genes involved in fibrosis, steatosis and inflammation (<i>Col1a1</i>, <i>Mmp9</i>, <i>Mmp2</i>, <i>α-SMA</i>, <i>Mmp13</i>, <i>Timp1</i>, <i>Casp1 and Tgfβ1</i>). Data are expressed as means ± SE. Statistical differences in gene expression were determined using a 2-way ANOVA to examine the main effects of maternal HFD and offspring MCD diet, followed by Student-Newman-Keuls post hoc analyses (*p<0.05, **p<0.01).