Additional file 2: Figure S2. of Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairment

Densitometry analysis of autophagic proteins in dose dependent (a) and time dependent (b) manner analyzed through western blot. GAPDH was served as a loading control. Values are expressed as mean Âą SE of three independent experiment. *p<0.05 was considered as statistical significant. (c) Real Time PCR for specificity of LC3 siRNA at gene level. (d) mRNA expression profile of control and GCNF exposed A549 cells for various genes involved in autophagy and apoptosis induction. The data represent the mean Âą SE of three independent experiment. *p