Additional file 5: Figure S5. of Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairment

GCNF induced autophagy mediated apoptosis in A549 cells. (a) Immunoblotting and respective densitometry analysis of apoptotic as well as autophagic protein in the presence of 3 – methyladenine (3 - MA) after GCNF (25 μg/ml) exposure in A549 cells. GAPDH was used as loading control. (b) Immunoblotting analysis for specificity of LC siRNA at protein level. (c) Immunoblotting and respective densitometry analysis of apoptotic as well as autophagic protein in the presence of LC3 siRNA after GCNF (25 μg/ml) exposure in A549 cells. GAPDH was used as loading control. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. (d) Viability of GCNF exposed cells was assessed using MTT assay in the presence or absence of 3-MA to check the role of autophagy in cell death. Values are expressed as mean ± SE of three independent experiment. *p