Inactivated-H5N1 increases phosphorylation of ERK1/2 and p38 MAPK.

<p>Immunoblot analysis of ERK1/2 <i>(A)</i> and p38 MAPK <i>(B)</i> protein expression in 16HBE14o- cells treated with inactivated-H5N1. Cells were untreated or treated with PD98059 (50 μM) or SB203580 (10 μM) as appropriate. One hour later, cells were treated for 30 min with vehicle, allantoic fluid or inactivated-H5N1 before being harvested for immunoblot analysis for the presence of ERK1/2, phospho-ERK1/2 (P-ERK1/2), p38 MAPK or phospho-p38 MAPK (P-p38). Upper panel, representative gels for immunoblot analysis of ERK1/2 and phosphorylated ERK1/2 (P-ERK1/2) <i>(A)</i> or p38 MAPK and phosphorylated p38 MAPK (P-p38) <i>(B)</i>. Lower panel, quantitative analysis of the amount of P-ERK1/2 (<i>A</i>) or P-p38 MAPK <i>(B)</i>, expressed as fold change, using ImageJ software (NIH). Values are means ± SEM from at least 3 sets of experiments. * and *** indicates <i>p</i> < 0.05 and <i>p</i> < 0.001, respectively (one-way ANOVA with Student-Newman-Keuls post-hoc test).