Plot of concentration profile similar to Fig 3 for all 12 KRAS mutations, based on experiment set 2.

<p>The profile is known to be linear, hence a single experiment per mutation type suffices. A higher slope corresponds to a stronger mutation. The bold dotted line is 10C→G (G12A in amino acid notation), the same mutation we used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0177384#pone.0177384.g003" target="_blank">Fig 3</a>. The horizontal dashed line corresponds to the threshold value <i>ρ</i>_<i>t</i> ≈ 0.5 from which we can derive the concentration threshold for each mutation.